Enlightening inhibitory neurotransmitter receptor organization and dynamics applying high-end microscopy

Project Description

The regulation of glycine receptor numbers and organization at synapses is essential for the efficacy of inhibition and the control of neuronal excitability in the spinal cord. Receptor biosynthesis, local recycling and diffusion controlled transport of receptors into and out of the synapse, which is influenced by synaptic clustering and scaffolding, represent important mechanisms underlying adaptations in the amount of neurotransmitter receptors present at synapses. Postsynaptic glycine receptor clusters were significantly reduced in both size and number in spinal cord neurons upon loss-of-function for the membrane trafficking and cytoskeletal effector syndapin I. We now aim at unraveling the molecular mechanisms underlying these defects in inhibitory neurotransmitter receptor trafficking and/or organization upon syndapin I loss-of-function applying high resolution light microscopy in order to be able to reveal postsynaptic receptor clusters and intracellular trafficking compartments that are below the resolution limit for conventional light microscopy.

Title Year Authors Journal Links
Fast structured illumination microscopy using rolling shutter cameras
2016 Song, L. Y., Lu-Walther, H. W., Forster, R., Jost, A., Kielhorn, M., Zhou, J. Y., and Heintzmann, R. Measurement Science and Technology More
Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength
2016 Lu-Walther, H.-W., Hou, W., Kielhorn, M., Arai, Y., Nagai, T., Kessels, M. M., Qualmann, B., Heintzmann, R. PLoS ONE More
Proteomic analysis of glycine receptor β subunit (GlyRβ)-interacting proteins: evidence for syndapin I regulating synaptic glycine receptors
2014 Del Pino, I., Koch, D., Schemm, R., Qualmann, B., Betz, H., and Paarmann, I. J Biol Chem More
Resolving a misconception about structured illumination
2014 Wicker, K., and Heintzmann, R. Nat Photon More
ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function
2014 Schneider, K., Seemann, E., Liebmann, L., Ahuja, R., Koch, D., Westermann, M., Hübner, C. A., Kessels, M. M., and Qualmann, B. J Cell Biol More
Optical Photon Reassignment microscopy (OPRA)
2013 Roth, S., Sheppard, C. J. R., Wicker, K., and Heintzmann, R. J Opt Nanosc More
Bayesian localization microscopy reveals nanoscale podosome dynamics
2012 Cox, S., Rosten, E., Monypenny, J., Jovanovic-Talisman, T., Burnette, D. T., Lippincott-Schwartz, J., Jones, G. E. and Heintzmann, R. Nat Methods More
The functions of the actin nucleator Cobl in cellular morphogenesis critically depend on syndapin I
2011 Schwintzer, L., Koch, N., Ahuja, R., Grimm, J., Kessels, M. M., and Qualmann, B. EMBO J More
Proper synaptic vesicle formation and neuronal network activity critically rely on syndapin I
2011 Koch, D., Spiwoks-Becker, I., Sabanov, V., Sinning, A., Dugladze, T., Stellmacher, A., Ahuja, R., Grimm, J., Schüler, S., Müller, A., Angenstein, F., Ahmed, T., Diesler, A., Moser, M., Tom Dieck, S., Spessert, R., Boeckers, T. M., Fässler, R., Hübner, C. A., Balschun, D., Gloveli, T., Kessels, M. M., and Qualmann, B. EMBO J More
Saturated patterned excitation microscopy (SPEM) - a novel concept for optical resolution improvement
2002 Heintzmann, R., Jovin, T. M., and Cremer, C. J Opt Soc Am A More
Syndapin isoforms participate in receptor-mediated endocytosis and actin organization
2000 Qualmann, B., and Kelly, R. B. J Cell Biol More
Lateral modulated excitation microscopy: Improvement of resolution by using a diffraction grating
1999 Heintzmann R., and Cremer, C. Proceedings of SPIE More